Transposon terminators

The Use of Terminators and Anti - Terminators to Expand the Two - Spatula

Prediction of transcription terminators in bacterial genomes.

This study describes an algorithm that finds rho-independent transcription terminators in bacterial genomes and evaluates the accuracy of its predictions. The algorithm identifies terminators by searching for a common mRNA motif: a hairpin structure followed by a short uracil-rich region. For each terminator, an energy-scoring function that reflects hairpin stability, and a tail-scoring functio...

Measurement and modeling of intrinsic transcription terminators

The reliable forward engineering of genetic systems remains limited by the ad hoc reuse of many types of basic genetic elements. Although a few intrinsic prokaryotic transcription terminators are used routinely, termination efficiencies have not been studied systematically. Here, we developed and validated a genetic architecture that enables reliable measurement of termination efficiencies. We ...

Mycobacterial RNA Polymerase Requires a U-Tract at Intrinsic Terminators and Is Aided by NusG at Suboptimal Terminators

Intrinsic terminators, which encode GC-rich RNA hairpins followed immediately by a 7-to-9-nucleotide (nt) U-rich "U-tract," play principal roles of punctuating and regulating transcription in most bacteria. However, canonical intrinsic terminators with strong U-tracts are underrepresented in some bacterial lineages, notably mycobacteria, leading to proposals that their RNA polymerases stop at n...

Illusory Contour-motion Arising from Translating Terminators

Periodic grating patterns were created by phase shifting or eliminating vertical columns of a fine line carrier grating oriented 45 deg. Motion was created by translating the patterns parallel to the carrier grating. This veridical motion was seen when terminators (i) were created in low-frequency carriers; (ii) terminated short lines; and (iii) moved slowly. In the complementary conditions an ...